Recombinant protein expression in Escherichia coli: advances and challenges

Escherichia coli is one of the organisms of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform....

Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli | Microbial Cell Factorie....

coli [1]. The main purpose of recombinant protein expression is often to obtain a high degree of accumulation of soluble product in the bacterial cell. This strategy is not always accepted...

Optimization of recombinant protein expression level in Escherichia coli by flow

Overexpression of recombinant proteins in Escherichia coli often leads to a severe growth retardation of the host cells. The phage T7 promoter phi10 in a pET vector was utilized to express human su...

Scholarworks@UNIST: Improving the Productivity of Recombinant Protein in Escheri

of Escherichia coli strains and facilitate the production of recombinant protein under thermal stress. The overexpression of... sustained in the second-round culture. The effect of GroELS...

Overcoming acetate in Escherichia coli recombinant protein fermentations

Escherichia coli is the organism of choice for the expression of a wide variety of recombinant proteins for therapeutic, diagnostic and industrial applications. E. coli generates acetic acid (aceta...

Industrial production of recombinant therapeutics in Escherichia coli and its recent advancements

Nearly 30% of currently approved recombinant therapeutic proteins are produced in Escherichia coli. Due to its well-characterized genetics, rapid growth and high-yield production, E. coli has been...

Expression of protein histidine phosphatase in Escherichia coli, purification, and determination of enzym....

A protein histidine phosphatase (PHP) from vertebrates was first identified in 2002. Here we describe the expression of that PHP in Escherichia coli and purification of the recombinant protein. In...

A comparison of inoculation methods to simplify recombinant protein expression s - Article 상세보기-Acces....

In the past five years, Structural Genomics (SG) initiatives have established an automated pipeline for protein production in Escherichia coli to rapidly screen various conditions, resulting in sol...

Biocatalytic properties of a recombinant Fusarium proliferatum lactonase with significantly enhanced prod....

25) was cloned and expressed in Escherichia coli JM109 (DE3) for biocatalytic resolution of... the recombinant protein, suggesting that the enzyme was a simplex protein. Furthermore...

Remaining Challenges for Recombinant Protein Production in Escherichia coli | Fr

Escherichia coli is the pioneering host for recombinant protein production. Its relatively easy genetic manipulation, rapid growth in inexpensive culture media, and high protein titers are the main...