The pipeline is particularly useful for the construction of combinatorial DNA assemblies. Furthermore, we demonstrate the platform by constructing a library of polyketide synthase parts...
In particular, recursive direction ligation by plasmid reconstruction (PRe-RDL) is the... expression plasmid, which is a low-to-medium copy number plasmid. While Pre-RDL can be used to...
Keywords ; high-throughput · molecular biology · plasmid-based · functional · proteomics · amino acid scanning · mutagenesis · integrated · automated robotic · workcell · metabolomics · synthetic biology
Highlights ; · pBI143 is a cryptic plasmid that is prevalent in industrialized human populations ; · pBI143 is only ∼2.7 kb, yet it regularly makes up over 0.1% of gut metagenomic reads ; · The naive 2-gene form appears parasitic, but pBI143 can carry additional genes
Plasmids are used extensively in basic and applied biology. However, design and construction of plasmids, specifically the ones carrying complex genetic information, remains one of the most time-co...
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(GUI) software. Furthermore, as seen commonly in the life sciences, reproducibility of DNA construction process descriptions is usually not guaranteed, and utilization of previously...
Restriction enzymes have long been used in plasmid construction, as they can cleave DNA... Vassily Andrew Petrov, CABBI Software Engineer; Roy Liu, CABBI undergraduate student in Computer...
We developed various plasmid cloning vectors that are useful in the construction of genomic and shotgun libraries. Two medium copy vectors, pCUGIblu21 (pCb21) and pAGIblu21 (pAb21), which...