Background Construction of plasmids is crucial in modern genetic manipulation. As of now, the common method for constructing plasmids is to digest specific DNA sequences with restriction enzymes an...
into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific...
Abstract Bacteria have existed on Earth for three billion years or so and have become adept at protecting themselves against toxic chemicals. Antibiotics have been in clinical use for a...
Berkeley Lab scientists have developed the first software package for automating DNA construction that not only makes the process faster and more efficient but - with an eye on the economics of sci...
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Abstract Plasmid shuttle vectors are a common tool used to study yeast physiology. The... Many studies throughout the published literature have included construction of yeast plasmids...
At Least One-Third of Zeros of Riemann's Zeta-Function are on σ = ½ ; Transplantation of Posterior Polar Plasm in Drosophila. Induction of Germ Cells at the Anterior Pole of the Egg ; Activity of Colicin E3-Treated Ribosomes in Initiation and in Chain Elongation
Although many methods have been reported, plasmid construction compromises transformant efficiency (number of transformants per ng of DNAs) with plasmid accuracy (rate of scarless plasmids). An eff...
Plasmid Construction Two sets of plasmids were used in the study, one containing SEAP and the other containing the mIL10 gene. The pLIVE-SEAP (renamed as pAlb-SEAPLIVE ) plasmid was...
A Plasmid-Borne Gene Cluster Flanked by Two Restriction-Modification Systems Enables an... , which could be utilized as the basis for the construction of genetic tools to facilitate both...