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NCBI is retiring the e-PCR tool effective immediately. The good news is that an existing tool, Primer-BLAST, fills in nicely for the functions of both Forward and Reverse e-PCR, and has the additio...
분자생물학실험 DNA EXTRACTION PCR TA Ligation E.coli transformation Mini-prep Sequence blast Restriction enzyme Mini-prep E.coli transformation TA Ligation PCR DNA EXTRACTION
MAX_TARGET_PER_TEMPLATE text 100 49 PRODUCT_MIN_TM text 50 PRODUCT_OPT_TM text 51 PRODUCT_MAX... 0 99 NEWWIN checkbox 100 SHOW_SVIEWER checkbox on Error in BLAST_primer(row$forward, row...
이는 Karlin-Altschul의 통계방법에 근거한 것이기 때문인데, cutoff 점수 S대신에 기대 cutoff E (expect ation cutoff E )를 대신 사용하기도 한다. 만일 기대 cutoff 점수를 사용하면 BLAST는 주어진 검색...
BLAST Workflow Template. Contribute to systemPipeR/SPblast development by creating an account on GitHub.
>P01013 GENE X PROTEIN (OVALBUMIN-RELATED) ; QIKDLLVSSSTDLDTTLVLVNAIYFKGMWKTAFNAEDTREMPFHVTKQESKPVQMMCMNNSFNVATLPAE ; KMKILELPFASGDLSMLVLLPDEVSDLERIEKTINFEKLTEWTNPNTMEKRRVKVYLPQMKIEEKYNLTS
-e Expectation value (E) [Real] 기본값 = 10.0 -o BLAST 결과 출력 파일 [File Out] 선택적. 기본값 = 표준출력stdout -F 질의서열 필터링 DUST with blastn, SEG with others) [String] 기본값 = TBLAST 2.0 과 2.1은 blastn 의...