Graphical abstract<ce:figure></ce:figure> AbstractDetermining the sequence specifity of DNA binding molecules is a non-trivial task. Here we describe the development of a platform for assaying the...
The nucleotide contains both a segment of the backbone of the molecule (which holds the chain together) and a nucleobase (which interacts with the other DNA strand in the helix). A...
We present single-molecule, real-time sequencing data obtained from a DNA polymerase performing uninterrupted template-directed synthesis using four distinguishable fluorescently labeled deoxyribon...
DNA can be sequenced by a chemical procedure that breaks a terminally labeled DNA molecule partially at each repetition of a base. The lengths of the labeled fragments then identify the positions o...
DNA labeling is a tool in molecular biology and biotechnology to visualize, detect, and study DNA at the molecular level. In this process, a DNA molecule is labeled by a set of specific patterns, r...
조규봉 · Kyubong Jo · 서강대학교 · #Single DNA Molecule · #Optical Mapping · #Genome Analysis · #DNA binding fluorescent proteins · #Nanopore Sequencing · #SEM ; 정원 · Won Jung · Harvard University · #Single-molecule tracking · #Super-resolution Imaging · #Protein-DNA interaction · #Gene expression regulation ; 장선복 · Sunbok Jang · 이화여자대학교 약학대학 · #단백질 · #BER · #NER · #Single molecule · #NMR · #X-ray Crystallography · #UV-DDB · #glycosylase
The formation and analysis of single molecules of fluorescently labeled DNA immobilized on polystyrene microspheres is described. Analysis by confocal fluorescence microscopy revealed single-step p...
DNA computing, the performing of computations using biological molecules, rather than traditional silicon chips. The idea that individual molecules (or even atoms) could be used for computation dat...
We have also shown that this technique can be extended to a multi color labeling scheme by using different dye labeled probes or by combining with a DNA- polymerase-mediated incorporation...
The labeled DNA molecules were then stretched into linear form on a modified glass surface and imaged using total internal reflection fluorescence (TIRF) microscopy. By determining the...